Microbes from the ocean


Prof David Smith gave a talk about microscopic life in the ocean.


Jenn Pietros: Today I got up and we put the CTD in the water.  This time it went down to 2000 meters!  I still cannot get over how vast the ocean is.  It is over a mile down to get to the bottom!  David Smith spent some time teaching us about how the salinity, temperature, density and fluorescence fluctuates throughout the water column and throughout the day.  We ended up taking samples of water from each of the bottles attached to the CTD and started conducting a mini experiment with the water.  We want to find out if marine bacteria change throughout the water column?  We took 12 different samples from various depths ranging from the surface down to 2000 meters.  We then used a pipette to put several drops into a 96 well plate used for growing bacteria.  We will check on these plates tomorrow to see what bacteria grow in the different wells.

Jess Grant Ecoplate Samples1

Jess Grant: What a great day! The first thing that I got to do when I woke up was take the lead with the first CTD cast of the day. I was able to communicate with the Winch Room and tell them what depths to deploy the CTD to. I was also able to fire the bottles of the CTD to collect different samples of sea water at different depths! Then we all went into the Wet Lab and dropped the samples into ecoplates that contained 96 holes in order to see which samples grew bacteria.


We took several samples of the water as the CTD slowly ascended back to the surface.  We used sterile containers to collect our water samples from the CTD and then worked together to fill up an EcoPlate for 6 different vials of water that were collected from 6 different depths.  The EcoPlate allowed us to run a microbial community analysis on each sample of water which will in time tell us what type of bacteria may living in our sample areas.


Alyssa Wood: David Smith introduced us to microbial community analysis.  Each ecoplate needed to be unwrapped since they are wrapped to keep sterile.  The plate has 96 pre-filled wells. Each plate repeats 31 carbon sources so in essence one plate contains three complete trials.  In each set there is a well of water as a control.  We learned that communities of organisms will give a characteristic reaction pattern called a metabolic fingerprint.


Joe Bartoshevich: Today aboard R/V Endeavor the morning started at 6:30 sharp with a beautiful sunrise. Then Frank, Meredith, Alyssa and I prepared the CDT and its bottles for deployment down to 1500 meters. Water samples would be taken at various depths. We are going to check for conductivity of the samples (the ones with more conductivity should contain more salt). We also took samples and using a pipette filled 96 samples holes which contain various substances such as sugars.Tomorrow we till see which ones reacted to each of the 96 substances.


Trisha Garland : After we recovered the CTD,   Lynn reviewed proper sterilization techniques with us and we took water samples from the 10L PVC cylinders. We’re trying to determine whether the same bacterial communities live at different depths of the water column. To do this, we put samples of water from different depths into well plates with 96 wells each. We had water samples from the surface, 80m, 100m, 200m, 1000m, and 2000m. Each well had a different substrate (a carbon/sugar) and an indicator (a salt). If the substrate in the well is metabolized, the water will turn purple. We’ll be checking on the plates tomorrow to see if the plates show any similarities.


Meredith Ashworth and Joe Bartoshevich begin analyzing samples.


Trisha and Aman looking for metabolic fingerprints.